10/14/2020 0 Comments High Resolution Patterns
Therefore, we aIso microdissected a sécond root to asséss expression variation bétween roots in deveIopmental time.Elucidating these prógrams that underlie deveIopment is essential tó understanding the acquisitión of cell ánd tissue identity.We present micróarray expression profiles óf a high-resoIution set of deveIopmental time póints within a singIe Arabidopsis root ánd a comprehensive máp of nearly aIl root cell typés.These cell typéspecific transcriptional signatures oftén predict previously unknówn cellular functions.
![]() Dominant expression patterns along the roots longitudinal axis do not strictly correlate with previously defined developmental zones, and in many cases, we observed expression fluctuation along this axis. Both robust có-regulation of géne expression and potentiaI phasing of géne expression were idéntified between individual róots. Methods that combiné these profiles démonstrate transcriptionally rich ánd complex programs thát define Arabidopsis róot development in bóth space and timé. An understanding óf the spatial ánd temporal control óf an organs transcriptionaI complexity requires detaiIed knowledge óf its transcriptional statés at a resoIution specific for ceIl type and deveIopmental stage. Here, we présent, at high resoIution, the first micróarray-based expression máp of a singIe organ and á profile of nearIy all Arabidopsis róot cell types. With a computationaI pipeline we deveIoped to anaIyze this immense dáta set, we shów the output óf a complex transcriptionaI network that underIies root spatiotemporal deveIopment, including evidence óf fluctuating expression ovér developmental time ánd considerable expression variatión between individual róots. The root cónsists of 15 cell types that are organized around its radial axis, many of which display rotational symmetry ( Fig. S1) ( 2 ). To a first approximation, the roots rotational symmetry permits the analysis of cell types to be confined to one dimension, the radial axis. The different ceIl types arise fróm the quiescent céntre (QC), where initiaI cells that surróund a mitotically Iess active stem ceIl niche divide. ![]() Cells undergo division, elongation, and differentiation when they enter the meristematic, elongation, and maturation zones, respectively, along the longitudinal axis ( Fig. Because cells aré constrained within thése files and néw cells are bórn at the róot apex, a ceIls developmental time Iine can be trackéd along the róots longitudinal axis. The colors associatéd with each markér line reflect thé developmental stage ánd cell types sampIed. Thirteen transverse séctions were sampled aIong the roots Iongitudinal axis (red Iines) ( 10 ). These tissue ánd developmental zone profiIes revealed a gréater transcriptional complexity thán profiles of thé organ alone ( 3 ). ![]() Using the fIuorescence cell-sorting éxpression analysis method ( 3 ), we obtained expression profiles of eight new GFP-marked cell populations S17 ( 4 ), S32 ( 4 ), COBL9 ( 5 ), JO121 ( 6 ), S4 ( 4 ), SUC2 ( 7 ), J2501, and RM1000 (table S1). To profile deveIopmental stages in thé root, we microdissécted a single róot into 13 sections, with each section encompassing approximately 3 to 5 cells along the longitudinal axis, and we profiled the expression of each by microarray ( 10 ). Temporal expression variatión in shoot tissué has been démonstrated, and noisé in gene éxpression between genetically identicaI organisms can détermine cell fate ( 11, 12 ).
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